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SELMA plate

SELMA plate

The figure shows two different SELMA plates under two different light conditions. Bacteria has not been cultivated on plates A and B, but on plates C and D, Staphylococcus aureus subsp. aureus has been cultivated. Plates A and C were photographed with a dark background and with lighting from above. Plates B and D were photographed with lighting from below. - Click on the image to enlarge it. - Click on the image to enlarge it.

 

 

SELMA plates are used for identification of bacteria, which may cause mastitis, and should only be used for milk samples. SELMA means SELective MAstitis medium and there are two variants of SELMA plates. SELMA, which has three sectors and SELMA PLUS, which has four sectors with different growth media.

On this page, only SELMA plates are shown and the sectors are numbered on the back of the plate. The sectors contain:

  1. Bovine blood agar with esculine where most aerobic (and facultatively anaerobic) bacteria grow.
  2. MacConkey agar where only gram negative bacteria grow.
  3. Mannitol salt agar where staphylococci and enterococci grow. Gram negative bacteria are inhibited by the high salt concentration. Mannitol salt agar can also be used to determine the production of penicillinase in staphylococci (se link below).

Note the double hemolysis of Staphylococcus aureus subsp. aureus can be observed on bovine blood agar with lighting from below. Decreased pH can be observed in the mannitol salt agar, where S. aureus subsp. aureus has been cultivated, but this bacterium does not grow on MacConkey agar.

For more information, see the SVA website, and "Användarinstruktion: Selma & Selma Plus", where the plates are observed from above.

Updated: 2017-08-30.

 

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Swedish University of Agricultural Sciences