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Species/Subspecies: Clostridioides difficile
Etymology: Genus name: an organism similar to members of the genus Clostridium.
Species epithet: difficult (refers to the difficulty to isolate it).
Significance:Nosocomial enteric infections caused by C. difficile in connection with antibiotic treatments of humans has recently attracted attention in the media in Sweden (Jan. 2009). C. difficile and calicivirus are the two most common nosocomial enteric pathogens in humans. A particularly aggressive strain (ribotype 027 or O27) of C. difficile has recently been demonstrated in patients in Sweden (January 2014). This strain is resistant against fluoroquinolones (gyrase inhibitors).
  [Very important]   
Old Species Name(s):Clostridium difficile
Type Strain: AS 1.2184 = ATCC 9689 = CCUG 4938 = NCTC 11209.
Macromorphology (smell):
Clostridioides difficile 
The large colonies (2-5 mm in diameter) have an irregular edge and fluoresces in green-yellow under UV light. Smells like horse manure. Gives no hemolysis on FAA plates.
Micromorphology: Large motile rods (0.5-1.9 x 3.0 to 16.9 µm) with peritrichous flagella. Can form spores.
Gram +/Gram -:
Fig. 26:3. Gram staining of Clostridioides difficile, strain CCUG 4938.

Metabolism: Strictly anaerobic
Other Enzymes: Lecithinase-, esculinase +, tryptophanase-.
Fermentation of carbohydrates:
Other carbohydrates: Fructose +, galactose -, mannose (+), ribose -, xylose (-).
Spec. Char.:
Hosts: Horse (foal), humans, pig etc.
Disease (Swedish):Antibiotika-associerad diarré (AAD) hos häst. Enterit hos spädgrisar. AAD och pseudomembranös kolit hos människa.
Disease (English):Antibiotic associated diarrhoea (ADD) in horses. Neonatal necrotizing colitis in pigs. ADD and pseudomambranous colitis in humans.
Clinical Picture:
Virulence Factors: Toxin A (enterotoxin) and toxin B (cytotoxin), which are encoded by the genes tcdA and tcdB, respectively.
Genome Sequence:
Acc-noStrainSize (bp)Genome
NC_009089 630 4 290 252 1c + 1c 

16S rRNA Seq.:
Acc-noStrainNumber of NTOperon
AB075770 ATCC 9689T 1 466 11 

 Clostridioides difficile
Only two species have been described within the genus Clostridioides, but this genus is related to the genus Clostridium, which consists of about 175 species. However, many species in this genus have proven to be incorrectly classified and C. difficile is an example of this.
Comment:Is a common environmental bacterium. Has also been found in animal foods.
Reference(s): No. 20, 33, 131
Link: Clostridia.net

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Swedish University of Agricultural Sciences