VetBact

Even if phenotypic methods are the most frequently used methods in diagnostic laboratories for determining antimicrobial resistance, genotypic methods connecting to identification of resistance genes have been developed. Genotypic (moecular) methods are usually based on conventional PCR (Polymerase Chain Reaction), real-time PCR or whole genome sequencing (WGS). These methods detect the presence of resistance genes or gene mutation that cause a certain resistance mechanism. The weakness of these methods is that they do not necessarily take into account whether the gene is expressed or not, and inaccurate clinical information could be generated.  

Updated: 2024-06-17.