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CCDA medium (blood-free selective medium for Campylobacter spp.)
The figure shows two CCDA plates. No sample has has been applied onto the plate in image A. Campylobacter sp. has been streaked onto the plate in image B. The colonies are greyish to white and get a metallic sheen if the plate is tilted in the light from a lamp, which can be seen on the CCDA plate on the C. jejuni subsp. jejuni page.
The medium, which is described here is actually a modified CCDA medium. CCDA plates contain a medium, which is used for selective isolation of Campylobacter spp. Cultivation on CCDA plates is performed at 37 or 42°C after surface spreading and incubation under a microaerophilic atmosphere in the presence of CO2 .
CCDA medium contains:
The final pH of the medium should be 7.4.
Campylobacter spp. colonies are round to irregular with smooth edges. They may have translucent, white colonies with a metallic sheen. Colonies of some strains my spread to a continous film (svarming) on moist agar.