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CCDA medium (blood-free selective medium for Campylobacter spp.)

CCDA agar plates

The figure shows two  CCDA plates. No sample has has been applied onto the plate in image A. Campylobacter sp. has been streaked onto the plate in image B. The colonies are greyish to white and get a metallic sheen if the plate is tilted in the light from a lamp, which can be seen on the CCDA plate on the C. jejuni subsp. jejuni page.

Applications

The medium, which is described here is actually a modified CCDA medium. CCDA plates contain a medium, which is used for selective isolation of Campylobacter spp. Cultivation on CCDA plates is performed at 37 or 42°C after surface spreading and incubation under a microaerophilic atmosphere in the presence of CO2 .

CCDA medium contains:

  • nutrient broth (nitrogen, vitamin and mineralsource)
  • casein hydrolysate (nitrogen, vitamin and mineralsource)
  • bacteriological charcoal (absorbs toxic substances and metabolites)
  • sodium deoxycholate (inhibits enteric bacteria, which are gram positive,  except Campylobacter spp.)
  • ferrous sulfate (iron(II) sulfate, which neutralizes toxic oxygen products)
  • amphotericin (inhibits yeast and fungi)
  • cefoperazone (inhibits enteric bacteria, which are gram negative,  except Campylobacter spp.)
  • sodium pyruvate (neutralizes toxic oxygen products)
  • agar (gelifying agent)
  • water

The final pH of the medium should be 7.4.

Summary

Campylobacter spp. colonies are round to irregular with smooth edges. They may have translucent, white colonies with a metallic sheen. Colonies of some strains my spread to a continous film (svarming) on moist agar.

Updated: 2017-02-24.

 

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