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Swedish University of Agricultural Sciences

Veterinary bacteriology: information about important bacteria
Veterinary bacteriology


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Cefsulodin-Irgasan-Novobiocin (CIN) agar

CIN agar plate

Applications:

CIN (Cefsulodin-Irgasan-Novobiocin) agar is a differential and selective medium used in qualitative procedures for the isolation of Yersinia enterocolitica from different clinical and nonclinical samples. Escherichia coli, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa, Salmonella enterica, Shigella sonnei and Streptococcus faecalis do not grow on CIN agar. By reducing the concentration of cefsulodin, CIN Agar could also be used for selective isolation of Aeromonas spp.

The medium contains:

  • mannitol
  • peptone (carbon and nitrogen source)
  • protease peptone (carbon and nitrogen source)
  • yeast extract (B-vitamin etc.)
  • sodium pyruvate (stimulates growth of Yersinia spp.)
  • NaCl (maintains the osmotic balance of the medium)
  • sodium deoxycholate (inhibits growth of gram positive and many gram negative bacteria)
  • sodium cholate (inhibits growth of gram positive and many gram negative bacteria)
  • neutral red (pH indicator)
  • MgSO4 (stimulates growth of Yersinia
  • Irgasan (inhibits gram positive and many gram negative bacteria)
  • Cefsulodin (inhibits the normal flora of enteric bacteria)
  • Novobiocin (inhibits the normal flora of enteric bacteria)
  • crystal violet (inhibits growth of gram positive and many gram negative bacteria)
  • agar (gelifying agent)
  • H2O

The final pH of this medium should be 7.4.

Other comments:

Differentiation of bacteria is based on mannitol fermentation. Organisms which are capable of fermenting mannitol will decrease the pH around the colony and the corresponding colour change of neutral red will generate colonis that appear as red. Because of the localized pH decrease, a zone of precipitated bile may also be present. The appearance of the colonies has some similarity with a "bull's eys". Organisms that do not metabolize mannitol to acid will form colorless, translucent colonies.

Updated: 2018-05-09.


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