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Mannitol egg Yolk Polymyxin agar (MYP agar)

Applications

Mannitol egg Yolk Polymyxin agar (MYP Agar) is a selective medium for detection and enumeration of Bacillus cereus in food samples. MYP Agar is also called MCS (Mossel Cereus Selective) agar or BCS (Bacillus Cereus Selective) Agar. MYP agar contains mannitol, which B. cereus does not ferment and egg yolk, which in turn contains lecithin that can be cleaved by the phospholipase C of B. cereus. Additionally MYP agar contains polymyxin, which inhibits gram negative bacteria.

MYP agar contains:

  • Meat extract
  • Pepton
  • Mannitol
  • NaCl
  • Phenol red
  • Agar
  • Water
  • The final pH of the medium should be 7.2. Eggyolk and polymyxin are aseptically added when the medium has been sterilized.

Summary

If B. cereus is growing on MYP agar a precipitate is formed around the colonies or the streak, and you will not get any yellowing because B. cereus does not ferment mannitol and there will be no decrease in pH around the colonies or the streak.

Updated: 2017-02-04.

 

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