Quick search:
Advanced search

Species/Subspecies: Escherichia coli
Etymology: Genus name: named after the German pediatrician Theodor Escherich, who first isolated the type species of the genus.
Species epithet: from the large intestine (colon).
Significance:  [Very important]   
Type Strain: ATCC 11775 = CCUG 29300 = NCTC 9001.
Macromorphology (smell):
Escherichia coli Escherichia coli Escherichia coli 
Medium sized (3-6 mm in diameter), opaque, sticky and greyish white colonies. Some strains give a narrow clear hemolysis zone on blood agar. Characteristic odor.
Micromorphology: Short motile rods (0.5 x 1-3 µm). E. coli is peritrichous, i.e. has flagella over the whole surface.
Gram +/Gram -:
Fig. 68:3. Gram staining of Escherichia coli, strain VB 008/14. The right image is a partial magnification (2.5x) of the left one. The total length of the respective scale bars is equivalent to 5 µm and the scale bars are placed in the corresponding areas of the images. Date: 2013-12-23.
Metabolism: Facultatively anaerobic.
Other Enzymes: DNase -, β-glucoronidase + (cf. Klebsiella spp.), esculinase V, ornithine decarboxylase V, tryptophanase + (= indol +), urease -.
Biochemical Tests: Citrate -, hydrogen sulphide (H2S) -, methyl red +, Voges-Proskauer -.
Fermentation of carbohydrates:
Both gas and acid are formed by those carbohydrates that are fermentable. There are biochemically inactive strains, which do not ferment some of the above carbohydrates.
Microbiological tests:
Escherichia coli 
Motility test is sometimes performed to confirm suspected findings of E. coli.
Spec. Char.:
Special Media:
Escherichia coli Escherichia coli Escherichia coli 
Escherichia coli Escherichia coli Escherichia coli 
BG medium is used to distinguish between E. coli and Salmonella spp. Chromogenic E. coli/coliform selective agar is used for enumeration of coliform bacteria. Doppsticka (t.ex. UriCult används i samband med urinvägsinfektioner). Dip s
Hosts: Many different animal species (including humans).
Disease (Swedish):Olika patotyper ger olika sjukdomar (enteriska, septikemiska m.fl.). Ödemsjuka hos gris (patotyp VTEC), avvänjningsdiarré hos gris (patotyp ETEC) och gulsäcksinflammation och äggledarinflammation hos fjäderfä.
Hemorragisk kolit hos människa (patotyp EHEC) (Matförgiftning).
Disease (English):Different pathotypes cause various diseases (enteric, septicaemic and others). Edema disease in pigs (pathotype VTEC), weaning diarrhea in pigs (pathotype ETEC) and yolk sac inflammation and salpingitis in poultry.
Hemorrhagic colitis in humans (pathotype EHEC) (Food Poisoning).
Clinical Picture:
Virulence Factors: Strains of E. coli are divided into  different pathotypes depending on their virulence factors. Some human patotypes and their virulence factors are listed below:

EPEC, enteropathogenic E. coli, type III secretion system (intimin, Tir, EspA, B, D och F) etc.
ETEC, enterotoxigenic E. coli, colonization factor antigens (CFAs), heat labile and heat stabile toxins (LT and STa, STB, respectively).
VTEC (= STEC), verotoxin producing E.coli, verotoxin.
EHEC, enterohemorragic E. coli, type III secretion system, Verotoxins (VT1 and VT2) etc.
EIEC, enteroinvasive E. coli, invasion plasmid (pINV).
EAggEC (=  EAEC), enteroaggregativ E. coli, aggregativa adhesion fimbriae (AAFs), AEAC flagellin and toxins.
UPEC, uropathogenic E. coli, adhesion factors (type 1, F1C, S, M and Dr), P fimbriae, cytotoxic necrotizing factor (CNF-1), hemolysin (HlyA) and autotransported protease (Sat).
MNEC, meningitis/sepsis-associated E. coli, outer membrane proteins (OmpA, IbA, IbeB, IbeC and AsIA), cytotoxic necrotizing factor (CNF-1) and capsule (K1).

Note that Shigatoxin is used synonymously with Verotoxin and the toxins are accordingly termed Stx1 and Stx2.

Genome Sequence:
Acc-noStrainSize (bp)Genome
AC_000091 K12-W3110 4 646 332 1c + 0 
Thirty-two strains have been sequenced including NC_002695, Stam O157:H7, 5 498 450 bp. These strains have up to 5 circular plasmids.

16S rRNA Seq.:
Acc-noStrainNumber of NTOperon
X80725 (T) 1450 

Legislation: E. coli, stam O157:H7, belongs to category B as a potential bioterrorism agent according to NIAID.
Comment:Type species for the genus. Most strains of E. coli are usually non-pathogenic intestinal bacteria, which belong to the normal flora, but some strains are pathogenic. Infections with verotoxin producing strains (VTEC) may be notifyable in Sweden.
Reference(s): No. 17, 40, 99, 124

Recently Updated

Recent blog posts

Recent comments